长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

中国鱼类名录ⅩⅢ——鲽形目、纯形目、海蛾鱼目、鮟鱇目     

李明德  张世禄王迎 《海洋通报》2003,22(6):56-62

鲽形目3亚目8科51属142种。鲀形目2亚目9科57属143种。海蛾鱼目1科2属3种，鮟鱇目3亚目11科23属41种。  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献   

The behaviour of ⁵⁹Fe in marine microhabitat     

Cai Fulong  Chen Ying  Xu Pi''an  Oiu Manhua 《海洋学报(英文版)》1989,8(3):423-429

Under the artificial condition the 59Fe morphology in sea water, its concentration in sediment and phytoplankton, its distribution and metabolism in tissue organs of marine animals were studied. The results showed that the morphology of 59Fe was in a particulate state in sea water. The adsorption rule of 59Fe by three kinds of sediments was similar. The concentration ability of 59Fe by phytoplankton was very strong. The critical concentration organs of S9Fe by marine animals were viscera. The gross radioactivity of 59Fe was mainly concentrated in protein. The concentration factor of 59Fe by DNA was the highest one. After excretion experiment, 59Fe of all the tissue organs was not detected. Small part of59Fe remained in the organic acid and protein state. There was a redistribution process in sediment for59Fe.  相似文献   

地形坡度对多金属结核分布的控制作用   总被引：3，自引：3，他引：3  

王英  李家彪  韩喜球  吴自银 《海洋学报》2001,23(1):60-65

选取我国东太平洋多金属结核开辟区内的一个区域作为研究区,利用人工神经网络中应用最为广泛的BP网络,建立控制多金属结核分布的地质因素与多金属结核分布之间的映射关系,探讨地形坡度对多金属结核分布的控制作用.结果表明,在经度、纬度、水深、坡度四个因素中,坡度对结核分布影响程度最大;多金属结核主要分布于坡度小于5°的地方;当坡度小于5°时,丰度与地形坡度呈正相关,品位与地形坡度呈负相关,丰度与品位呈负相关;品位与坡度似呈指数关系,坡度增大,品位降低.  相似文献   

栉孔扇贝升温育苗高产技术   总被引：1，自引：0，他引：1  

于瑞海  徐承阳 《海洋湖沼通报》1992,(2):68-71

在栉孔扇贝升温育苗中,主要改进了亲贝升温促熟培育、产卵与孵化、选幼、幼虫培育等主要技术措施,在105m~3水体中,培育出商品苗85,000,000多粒,取得了可喜的经济效益。  相似文献   

壳聚糖酶高产菌株的筛选和发酵条件的研究   总被引：6，自引：0，他引：6  

孙玉英  韩宝芹  刘万顺  张继泉  姚如永  彭睿 《中国海洋大学学报(自然科学版)》2007,37(2):266-272

从采集的土样中分离到1株产壳聚糖酶能力较强的菌株OU01,并对OU01进行了16S rDNA序列、形态及生理生化鉴定分析。16S rDNA序列分析表明该菌属于微杆菌属;该菌的形态特征与生理生化鉴定结果表明该菌与Microbacteriumsp.的相似性最高;因此将其初步鉴定为微杆菌属(Microbacterium)。同时对该菌的发酵条件进行了初步研究:该菌的最适培养基组成为(g/L):chitosan 10,(NH4)2SO420,MgSO4.7H2O 1.3,K2HPO4.3H2O 1.4,Glucose 1,Yeast extract 3,NaCl 5,起始pH=6.30。最适发酵温度为30℃,最佳发酵时间为96 h,在上述最优条件下,该菌株产酶达到118 U/mL。Microbacteri-umsp.OU01菌株为壳聚糖酶的研究和应用提供了新的来源。  相似文献   

胶州湾大沽河口洪水期三维水沙数值模拟研究     

韩树宗  赵瑾  魏福宝  林俊 《中国海洋大学学报(自然科学版)》2007,37(5):689-694

大沽河是胶州湾最大的入海河流,每年都会给胶州湾带来大量泥沙。本文利用三维的ECOMSED水动力-泥沙数值模型对洪水期的大沽河进行了数值模拟,得到了洪水存在期间以及洪水结束后胶州湾的余流分布、大沽河河道上中下游各层盐度、泥沙浓度随时间变化的趋势以及入海冲淡水和泥沙对胶州湾的影响。  相似文献   

Research on Characteristics of Density Current Under the Action of Waves     

Li Dashan  Shen Ying  Ren Rushu Chen Yao Senior Engineer  River  Harbour Department  Nanjing Hydraulic Research Institute  Nanjing Engineer  River  Harbour Department  Nanjing Hydraulic Research Institute  Nanjing Associate Professor  Hohai University  Nanjing Master  Hohai University  Nanjing 《中国海洋工程》1997,(1)

In this paper,the characteristics of density current under the action of waves are describedwith the help of flume experiment and theoretical analysis.The study shows that turbid water under the ac-tion of the waves can present three types of motion,i.e.significant stratification,fragile stratification andstrong mixing.The motion of turbid water presents significant stratification when(H/D)/△ρ/ρ~(1/2)≤4.5,generally this state is known as density current.The formulas of motionvelocity,thickness,and discharge of density current moving on horizontal bottom are derived by use of ba-sic equations such as momemtum equation,equation of energy conservation and continuity equation offluid.The time-average velocity and the thickness of density current under the action of waves have a rela-tionship with such parameters as relative density(△ρ/ρ),wave height(H),and water depth(D).Whenthese parameters are determined,the time-average thickness and motion velocity of density current are al-so determined.The relat  相似文献   

The impact of several chemical poisons on the absorbing ⁴⁵Ca by the Arca granosa     

Cai Fulong  Chen Ying  Huang Lingyi 《海洋学报(英文版)》1992,11(3):435-440

The impact of several chemical poisons on the Arca granosa absorbing 45Ca was studied. The results showed that the absorption of 45Ca in the blood and the soft tissue by Arca granosa was inhibited by the inorganic compounds of Cu, Zn, Pd, Cd, Hg and so on, among which the inhibition of Cd was the strongest, next was that of Cu and Hg. But the inhibition of the absorption of 45Ca in the shell of Arca granosa was little. The impact of three kinds of energy metabolism inhibitory substances on the absorption of 45Ca by the Area granosa showed that the absorption was an active transfer process.  相似文献